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Common peroneal nerve entrapment during closed reduction and percutaneous pinning of paediatric distal femur fracture: Surgeons be aware!

Volume 4 | Issue 1 | January-June 2018 | Page: 38-40 | Kiran Sasi, Binu P Thomas DOI- 10.13107/ijpo.2018.v04i01.009 Authors: Kiran Sasi, Binu P Thomas Department of Hand Surgery, Dr. Paul Brand Centre for Hand Surgery and Peripheral Nerve Surgery, Christian Medical College and Hospital, Vellore, Tamil Nadu, India. Address of Correspondence Dr. Binu P […]

Isolation, in-vitro expansion, and characterization of human muscle satellite cells from the rectus abdominis muscle

Volume 4 | Issue 1 | January-June 2018 | Page: 16-22 | David Livingstone, Albert A Kota1, Sanjay K Chilbule, Karthikeyan Rajagopal, Sukria Nayak, Vrisha Madhuri DOI- 10.13107/ijpo.2018.v04i01.005 Authors: David Livingstone, Albert A Kota [1], Sanjay K Chilbule, Karthikeyan Rajagopal, Sukria Nayak [1], Vrisha Madhuri Department of Orthopaedics, Paediatric Orthopaedics Unit, 1Department of Surgery, […]

Common peroneal nerve entrapment during closed reduction and percutaneous pinning of paediatric distal femur fracture: Surgeons be aware!

Isolation, in-vitro expansion, and characterization of human muscle satellite cells from the rectus abdominis muscle

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Common peroneal nerve entrapment during closed reduction and percutaneous pinning of paediatric distal femur fracture: Surgeons be aware!

Volume 4 | Issue 1 | January-June 2018 | Page: 38-40 | Kiran Sasi, Binu P Thomas

DOI- 10.13107/ijpo.2018.v04i01.009

Authors: Kiran Sasi, Binu P Thomas

Department of Hand Surgery, Dr. Paul Brand Centre for Hand Surgery and Peripheral Nerve Surgery, Christian Medical College and Hospital, Vellore, Tamil Nadu, India.

Address of Correspondence
Dr. Binu P Thomas,
Dr. Paul Brand Centre for Hand Surgery and Peripheral Nerve Surgery, Christian Medical College and Hospital, Vellore – 632 004, Tamil Nadu, India.
E-mail: binu@cmcvellore.ac.in

Abstract

Distal femoral metaphyseal fracture is a common injury faced by paediatric orthopaedic surgeons. This injury is usually managed with closed reduction and percutaneous Kirschner wire fixation. We present an unusual case wherein the common peroneal nerve was completely severed and entrapped in the fracture site following closed reduction and percutaneous Kirschner wire fixation of a distal femoral metaphyseal fracture.
Keywords: Distal femur fracture, Foot drop, Nerve entrapment

References

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How to Cite this Article: Sasi K, Thomas BP | Common peroneal nerve entrapment during closed reduction and percutaneous pinning of paediatric distal femur fracture: Surgeons be aware! | January-June 2018; 4(1): 38- 40.

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Isolation, in-vitro expansion, and characterization of human muscle satellite cells from the rectus abdominis muscle

Volume 4 | Issue 1 | January-June 2018 | Page: 16-22 | David Livingstone, Albert A Kota1, Sanjay K Chilbule, Karthikeyan Rajagopal, Sukria Nayak, Vrisha Madhuri

DOI- 10.13107/ijpo.2018.v04i01.005

Authors: David Livingstone, Albert A Kota [1], Sanjay K Chilbule, Karthikeyan Rajagopal, Sukria Nayak [1], Vrisha Madhuri

Department of Orthopaedics, Paediatric Orthopaedics Unit, 1Department of Surgery, Unit IV, Christian Medical College, Vellore, Tamil Nadu, India

Address of Correspondence
Dr. Vrisha Madhuri,
Paediatric Orthopaedics Unit, Christian Medical College, Vellore – 632 009, Tamil Nadu, India.
E-mail: madhuriwalter@cmcvellore.ac.in

Abstract

Introduction: Satellite cells are a resident population of stem cells beneath the basal lamina of mature skeletal muscle fibers. Their capacity to regenerate muscle makes them a potentially ideal source for human cell therapy with respect to muscle-related diseases such as urinary and fecal incontinence, and others. In this study, we describe a protocol to isolate, expand in-vitro, and characterize human muscle satellite cells from the rectus abdominis muscle. Materials and Methods: Muscle biopsies from human donors were harvested, digested using collagenase type II, and then plated on extracellular matrix-coated plates.
Results: Immunocytochemistry revealed that satellite cells on day 8 were 70–80% Pax7 positive; in contrast, cells expanded until day 12 showed 50–75% positivity for Pax7. The real-time polymerase chain reaction for day 8 culture indicated four-fold increase in Pax3 and Pax7 gene expression, four-fold increase in MyoD gene expression, and five-fold increase in Myf5 gene expression.
Conclusion: These findings suggest that satellite cells can be cultured until day 8 for translational purposes. The protocol described here is modest, operational, and reproducible and involves only basic cell culture equipment.
Keywords: Cell therapy, Human skeletal muscle, Myoblast, Satellite cells, Sphincter injuries, Tissue regeneration

References

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How to Cite this Article: Livingstone D, Kota AA, Chilbule SK, Rajagopal K, Nayak S, Madhuri V | Isolation, in-vitro expansion, and characterization of human muscle satellite cells from the rectus abdominis muscle | January-June 2018; 4(1): 16-22.

Half Sized Blog Element (Single Author Style)

Half Sized Blog Element (Multi Author Style)

Common peroneal nerve entrapment during closed reduction and percutaneous pinning of paediatric distal femur fracture: Surgeons be aware!

Isolation, in-vitro expansion, and characterization of human muscle satellite cells from the rectus abdominis muscle

Common peroneal nerve entrapment during closed reduction and percutaneous pinning of paediatric distal femur fracture: Surgeons be aware!

Isolation, in-vitro expansion, and characterization of human muscle satellite cells from the rectus abdominis muscle

Full Sized Blog Element (Big Preview Pic)

Common peroneal nerve entrapment during closed reduction and percutaneous pinning of paediatric distal femur fracture: Surgeons be aware!

Authors: Kiran Sasi, Binu P Thomas

Abstract

References

(Article Text HTML) (Download PDF)

Isolation, in-vitro expansion, and characterization of human muscle satellite cells from the rectus abdominis muscle

Authors: David Livingstone, Albert A Kota [1], Sanjay K Chilbule, Karthikeyan Rajagopal, Sukria Nayak [1], Vrisha Madhuri

Abstract

References

(Article Text HTML) (Download PDF)

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